TY  - THES
T1  - The potential of nanoscale carriers for drug delivery to intestinal mucosa and skin
A1  - Weiß,Barbara
Y1  - 2007/05/08
N2  - Subjects of the present thesis were the formulation and evaluation of poly(lactide-co-glycolide) (PLGA) based nanoparticles (NP) addressing the biological barriers intestine and skin. As first step, fluorescence labelled PLGA NP were developed and characterized to study and to visualise interactions with such anatomical sites. Stable fluorescence labelling was accomplished by a covalent polymer modification with a fluoresceinamine. The potential of these NP to investigate penetration and storage in hair follicles in vitro and in vivo was examined within the scope of this work. Moreover, they were evaluated to represent powerful tools to study accumulation and retention in inflamed intestinal mucosa in inflammatory bowel diseases in future clinical studies. Then, the fluorescence labelled NP were advanced to dually fluorescence labelled NP by incorporation of Texas Red as fluorescent model drug. Those NP were applied to demonstrate the impact of multiphoton microscopy to simultaneously study penetration and drug release on excised human skin. Employing flufenamic acid as hydrophilic model drug, the influence of nanoencapsulation on drug penetration into skin was studied using PLGA NP as drug carriers. Finally, a technique to surface functionalize preformed PLGA NP was developed. This approach may allow subsequent versatile binding of proteins (targeting moieties, drugs) and dyes for various applications of interest e.g. targeting the intestinal mucosa.
KW  - Nanopartikel
KW  - Fluoreszenzmarkierung
KW  - Biotin
KW  - Haut
KW  - Darmschleimhaut
KW  - In vitro, In vivo
CY  - Saarbrücken
PB  - Universitäts- und Landesbibliothek
AD  - Postfach 151141, 66041 Saarbrücken
UR  - http://scidok.sulb.uni-saarland.de/volltexte/2007/1116
ER  -