TY  - THES
T1  - Proteome analysis of Schizosaccharomyces pombe using two-dimensional gel electrophoresis and mass spectrometry : examination of 11-deoxycorticosterone (DOC) induced differential protein patterns
A1  - Hwang,Kyung Hoon
Y1  - 2008/01/08
N2  - The overall aim of this work was the investigation of the proteom of the fission yeast Schizosaccharomyces pombe (S. pombe) by two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS). The first goal of this study was to establish a two-dimensional gel electrophoresis reference map (2-D reference map) for proteins of S. pombe wild type strain h-S L 972. The benefit of 2-D reference maps is that they can provide much information on the expression, function and regulation of proteins and a survey of proteins affected during different physiological processes. In this study 364 proteins have been identified by MS approaches, amongst others two membrane proteins. After subsequent database searches, 157 out of these 364 distinct proteins could be functionally classified. 
The aim of the second part was to identify proteins, which are altered in S. pombe due to the mineralocorticoid receptor (MR) -independent action of the steroid hormone 11-deoxycorticosterone (DOC). The fission yeast S. pombe was chosen since it does not contain nuclear steroid receptors. Investigation of this subject was performed by combining two dimensional electrophoresis, PDQuest software and MS. By using specific Analysis Sets in PDQuest, a total number of 42 spots from silver stained gels displaying significant intensity differences between the samples treated with DOC and control samples were visualized. After MS analysis, 19 distinct proteins have been identified.
KW  - Schizosaccharomyces pombe
KW  - Proteomanalyse
KW  - Gelelektrophorese
KW  - Massenspektrometrie
KW  - Proteinmuster
CY  - Saarbrücken
PB  - Universitäts- und Landesbibliothek
AD  - Postfach 151141, 66041 Saarbrücken
UR  - http://scidok.sulb.uni-saarland.de/volltexte/2008/1408
ER  -