TY  - THES
T1  - Fluorescence correlation spectroscopy of autofluorescent proteins and its applications in live cell membranes
A1  - Koyadan Veettil,Seena
Y1  - 2009/07/09
N2  - Fluorescence correlation spectroscopy (FCS) is used for investigating the kinetic processes and molecular interactions by means of the statistical analysis of fluorescence fluctuations. These fluorescence fluctuations which arise from the small confocal volume are due to diffusion, rotation, intersystem crossing, conformational changes or other random effects of the fluorescent molecules under study. Therefore the fluorescent intensity from these fluctuations contains photophysical information on the nature of molecules. The aim of the work described in this thesis is to study the applications of FCS. The thesis is divided into 6 chapters. The theory, experimental setup and applications of FCS are outlined in chapter 1. A method of determining the photostability of autofluorescent proteins by Stern-Volmer plot like analysis is described in chapter 2. Photostability of green (GFP) and yellow fluorescent proteins (YFP) with fluorinated chromophores are analysed by intensity dependent FCS measurements. Such a photostability analysis is continued in chapter 3 with F64L containing GFP mutants. In addition, this chapter also explains a thermal relaxation rate constant of EGFP which is accompanied by the transition between the bright state and the dark state. By wavelength dependent FCS measurements, the protonated state of the Thr203 and Glu222 containing GFP mutants are demonstrated in chapter 4. Chapter 5 describes the effect of membrane potential on the lateral diffusion of the K+(Na+)/H+ exchanger on the membrane of live red blood cell (RBC). Characterization of PLGA (polylactic-co-glycolic acid) nanoparticles tagged with oligonucleotides which are potential drug carriers in pharmaceutical applications are studied in chapter 6.
KW  - Fluoreszenzkorrelationsspektroskopie
KW  - Grün fluoreszierendes Protein
KW  - Einzelmolekülspektroskopie
CY  - Saarbrücken
PB  - Universitäts- und Landesbibliothek
AD  - Postfach 151141, 66041 Saarbrücken
UR  - /2009/2196
ER  -