TY - THES T1 - Reactive fluorescent dye systems for single-molecule studies A1 - Schmitt,Alexander Y1 - 2009/10/30 N2 - Single-molecule spectroscopy is a well established method in physics and life sciences. Appliances on mechanistic problems in chemistry are rare, which can be traced back to the lack of suitable fluorophores. Therefore the development of a reactive system for future imaging of chemical reactions on a single-molecule level had top priority in this work. Dye systems based on the BODIPY fluorophore were prepared. For the first time, the synthesis of the core compound of this dye family is presented here. Methylated derivatives of this core compound have been characterized concerning their photostability and were categorized as superior to fluorescein. Elementary photophysical processes have been examined for their suitability as read-out mechanism for the project aim. Here a substrate for phosphorester cleavage has been developed where the reaction could be detected by a change of fluorescence lifetime. Another read-out mechanism was based on the change of fluorescence colour by chemical reactions. Appropriate substrates for oxidation reactions and metathesis reactions could be developed. The systems are promising candidates for the projected pursuit of individual reaction processes by ultrasensitive fluorescence spectroscopy. KW - Einzelmolekülspektroskopie KW - Fluoreszenzfarbstoff KW - Dihydroxylierung KW - Metathese CY - Saarbrücken PB - Universitäts- und Landesbibliothek AD - Postfach 151141, 66041 Saarbrücken UR - http://scidok.sulb.uni-saarland.de/volltexte/2009/2530 ER -