TY  - THES
T1  - Expression of human steroid hydroxylases in fission yeast
A1  - Dragan,Calin-Aurel
Y1  - 2010/12/20
N2  - Genetically modified microbial organisms are being increasingly used for the industrial production of complicated chemical compounds such as steroids; however, prior to this work there have been few reports on the use of the fission yeast Schizosaccharomyces pombe for this purpose. In the human adrenal, the mitochondrial P450 enzyme CYP11B1 catalyzes the conversion of 11-deoxycortisol to cortisol while the microsomal P450s CYP17A1 and CYP21A1 together with the aldo-keto reductase 3beta-HSD perform the crucial conversion of pregnenolone to either mineralocorticoid, glucocorticoid, and sex steroid precursors. Expression of these P450s in fission yeast resulted in strains that show a considerable biotransformation activity in whole-cell assays carried out over several days. The host is capable of supplying this enzymes with the reducing equivalents necessary for steroid hydroxylation activity indifferent of whether the P450s are localized in mitochondria or in the endoplasmic reticulum.
KW  - Steroide
KW  - Cytochrom P-450
KW  - Heterologe Genexpression
KW  - Schizosaccharomyces
KW  - Biotransformation
CY  - Saarbrücken
PB  - Universitäts- und Landesbibliothek
AD  - Postfach 151141, 66041 Saarbrücken
UR  - http://scidok.sulb.uni-saarland.de/volltexte/2010/3485
ER  -