TY  - THES
T1  - Transport of metal oxide nanoparticles across the human air-blood barrier : interactions with physiologically relevant media and proteins
A1  - Schulze,Christine
Y1  - 2011/02/07
N2  - There is still a big gap between nanoparticle production and industrial use on one hand and the knowledge of their toxicological potential on the other. In vitro assays are a common tool to investigate toxicity of substances, but for nanoparticles, some especially dispersion related pitfalls must be recognized and bypassed prior to correct interpretation of results. One special feature of nanoparticles is the possible interaction with proteins in cell culture media and with physiological proteins as well. On one hand, those interactions can have an influence on the agglomeration state, on the other hand cell reactions and hence the toxicological potential can be altered. 

Investigations with the model protein BSA or FCS, respectively, revealed differences for the adsorption onto nanoparticles, although the particles tested had very similar physico-chemical properties. BSA seemed to adsorb to the particles in different conformations, and the state of agglomeration must be taken into account to draw conclusions about protein adsorption.
Protein adsorption was also confirmed for physiologically relevant Surfactant protein A to eight different nanoparticles of partially the same bulk material. Also here, differences in protein adsorption could be detected. In contrast to BSA, Sp-A does not have much impact on the agglomeration state of the particles.
Inhaled particles might cross the air-blood barrier and enter the blood stream. Hence, an in vitro air-blood barrier model was adapted to transport experiments with nanoparticles. The cell culture conditions were adapted and the transport characteristics of the cells confirmed. Except two different CeO2 particles, no metal oxide nanoparticle transport could be detected.

KW  - Lunge
KW  - Nanopartikel
KW  - Surfactant-Protein A
KW  - In vitro
CY  - Saarbrücken
PB  - Universitäts- und Landesbibliothek
AD  - Postfach 151141, 66041 Saarbrücken
UR  - http://scidok.sulb.uni-saarland.de/volltexte/2011/3535
ER  -