TY  - THES
T1  - Iron regulation in the myxobacterium Myxococcus xanthus DK1622
A1  - Altmeyer,Matthias O.
Y1  - 2011/02/23
N2  - The myxobacterium Myxococcus xanthus DK1622 is a reliable producer of different secondary metabolites with partially unknown bioactivities. In the present work the response of iron availibility were evaluated, concerning effects on growth, proteome profile and secondary metabolite production. The production of the siderophore myxochelin A was increased by the factor 81, myxochelin B by the factor 678. Unexpectedly, several other secondary metabolite production rates were found influenced, as e.g. myxochromids und cittilins. In proteome analysis, 1979 protein spots were detected in average, whereof 172 exhibited an iron-induced change in expression. A subsequent analysis by tandem mass spectrometry identified 169 of these spots as 131 individual proteins, some with up to 3 protein-phosphorylations. Furthermore, the functions of some, interesting proteins were investigated by knockout of the respective coding gene. At all, 12 single crossover mutants were generated and compared in iron-rich environment concerning effects on growth and rates of iron uptake or secondary metabolite production to the wild type strain. Typically, mutant strains show variations in all three parameters. An in-frame deletion mutant in one of the two fur genes (MXAN_6967) exhibited reduced growth and a decrease in iron uptake (ca. 49 % of the wild type). Additionally, production of all seven monitored secondary metabolites cannot be explained with the traditional Fur model, which suggests a new, unexpected regulation in M. xanthus.
KW  - Proteomanalyse
KW  - Eisen
KW  - Sekundärmetabolit
KW  - Myxococcus xanthus
KW  - Myxobakterien
CY  - Saarbrücken
PB  - Universitäts- und Landesbibliothek
AD  - Postfach 151141, 66041 Saarbrücken
UR  - http://scidok.sulb.uni-saarland.de/volltexte/2011/3545
ER  -