TY  - THES
T1  - Effect of transmembrane potential on the diffusion of Na⁺/H⁺ exchanger of human red blood cell
A1  - Pasula,Aravind
Y1  - 2011/04/04
N2  - The RBC membrane differs from a simple bilayer membrane by its mechanical properties, shear viscoelasticity and by the long range mobility of integral membrane proteins. The influence of transmembrane potential of human RBCs on the lateral diffusion of Na⁺/H⁺ exchanger and membrane lipid analogue Bodipy-HPC has been studied using fluorescence correlation spectroscopy (FCS). Variable changes in the diffusion constants of Na+/H+ exchanger at different transmembrane potentials and corresponding cytoskeleton interactions have been explained. The role of volume changes of RBCs on the diffusion constant of Na⁺/H⁺ has been studied. The obtained data suggest that the transmembrane potential has no significant influence on the lateral diffusion of lipid analogue Bodipy-HPC. Additionally, the mechanism behind the Ca²⁺ loss of a single Caco-2 cell in physiological conditions has been studied in detail. The mechanism behind such loss and the suitable inhibitor to block this loss has been studied. Different inhibitors for Ca²⁺ channels and pumps have been used to understand the responsible mechanism has been found out. It has been demonstrated, that the detached single Caco-2 cell, from the epithelium, loses Ca²⁺ through L-type channels. Moreover, the influence of nano-structured surfaces and nano-particles on the physiological processes like Ca²⁺ transport and intracellular pH of RBCs and Caco-2 cells has been studied. Changes in the intracellular pH and Ca²⁺ transport on living cells have impact on the cell metabolism and physiology. It has been shown that most of the surfaces with various patterns and textures on the glass surface do not influence the Ca²⁺ transport and intracellular pH. Polymer surfaces with different precursor material other than glass (borosilicate) have shown to exert significant influence on both Ca²⁺ transport and pH of RBCs and Caco-2 cells.
KW  - Blutzelle
KW  - Erythrozyt
KW  - Membranproteine
KW  - Diffusion
CY  - Saarbrücken
PB  - Universitäts- und Landesbibliothek
AD  - Postfach 151141, 66041 Saarbrücken
UR  - http://scidok.sulb.uni-saarland.de/volltexte/2011/3747
ER  -