TY  - THES
T1  - Novel analysis approaches to context-dependent molecular networks
A1  - Emig,Dorothea
Y1  - 2011/04/14
N2  - Proteins are key players in all kinds of biological processes and accurate knowledge of their presence and their interactions is fundamental for understanding the functioning of the cells. Over the last years, many large-scale studies have been performed in order to unravel the complete human interactome. However, the results of these studies usually depend on the cellular conditions, in which the protein interactions were detected. Furthermore, additional biological mechanisms or temporal and spatial constraints contribute to the context-dependent formation of protein interactions.

In this thesis, we focus on different biological aspects that are important for the formation of protein-protein interactions. We first analyze protein interactions in a structural context and demonstrate that interacting proteins may collide in three-dimensional space, rendering the interaction impossible. Second, we investigate the tissue-specific formation of protein interactions. We analyze the ability of different technologies such as microarray platforms and next-generation RNA-sequencing to reliably detect tissue-specific gene expression. We further use gene expression data to identify tissue-specific protein interactions and their functional implications. Finally, we concentrate on protein variants that arise by alternative splicing events. We describe our software DomainGraph that allows for visually exploring protein variants and their interactions in different biological conditions.
KW  - Bioinformatik
KW  - Netzwerk
KW  - Genexpression
KW  -  RNS-Spleißen
KW  - Proteine
CY  - Saarbrücken
PB  - Universitäts- und Landesbibliothek
AD  - Postfach 151141, 66041 Saarbrücken
UR  - http://scidok.sulb.uni-saarland.de/volltexte/2011/3843
ER  -