TY  - THES
T1  - Multilayer coating of gold nanoparticles (AuNP) with drug-polymer complex : development and characterization
A1  - Reum,Nico
Y1  - 2011/07/12
N2  - Photosensitizers (PS) in combination with visible light are approved in photodynamic therapy for treatment of several types of cancer. Recent strategies for higher bioavailability and the reduction of side effects include PS loaded nanoparticle formulations. The subject of the present thesis is the development and characterization of gold based and surface modified nanoparticulate drug delivery systems for the PS 5,10,15,20-tetrakis(3-hydroxyphenyl)chlorin (mTHPC) for intravenous administration. Gold nanoparticles (AuNP) with a diameter of 15 nm were prepared and used as template for the Layer-by-Layer (LbL) approach. The PS and the negatively charged polyelectrolyte (PE) poly(styrene sulfonate) sodium salt (PSS) were complexed with a new developed method using freeze-drying resulting in a dramatically increased water solubility of the PS. The phototoxicity of the PSS/mTHPC complex in water was increased compared to the free mTHPC in ethanol. A drug-multilayer system based on the LbL technique utilized the water-soluble complex as anionic layer material and poly(allylamine hydrochloride) (PAH) as cationic layer. For the first time, a nanoparticulate system with three adsorbed drug layers was prepared. The mTHPC-loaded modified AuNP were taken up by cells and the dark toxicity was strongly decreased. The mTHPC was released and effective as anticancer drug after illumination. In conclusion, the modified AuNP can be considered as promising carrier for such types of PS.
KW  - Nanopartikel
KW  - Therapeutisches System
KW  - Photosensibilisator
KW  - Photodynamische Therapie
KW  - Polyelektrolyt
CY  - Saarbrücken
PB  - Universitäts- und Landesbibliothek
AD  - Postfach 151141, 66041 Saarbrücken
UR  - http://scidok.sulb.uni-saarland.de/volltexte/2011/4208
ER  -