TY  - THES
T1  - Exploiting the natural products of novel myxobacteria : phylogenetic and fatty acid perspectives and bioactive compound discovery
A1  - Garcia,Ronald O.
Y1  - 2011/09/08
N2  - Myxobacteria synthesise diverse and interesting secondary metabolites with impressive biological activities and modes of action. In an effort to uncover new bioactive compounds, world-wide samples were explored using various cultivation and isolation techniques. Several novel isolates were successfully cultivated representing a new family (Phaselicystidaceae) and new genera (Phaselicystis, Aetherobacter, Minicystis, Pseudochondromyces). Based on 16S rRNA gene sequence analysis, they appear to represent the so-far "uncultivated'; group of myxobacteria. During the chemical characterisation of novel isolates, large quantities of commercially valuable polyunsaturated fatty acids (PUFAs) were detected by GC-MS analysis. Eight PUFAs, comprising different ω-3 and ω-6 fatty acids (FAs), were identified for the first time in myxobacteria. Based on FA and 16S rRNA gene analyses, myxobacteria were again proven to be a coherent group. This finding not only pioneers the chemo-phylogenetic correlation of myxobacteria but also aids in the identification of potentially new PUFA-producing strains. The study also highlights the discovery of new bioactive compounds in the novel species Aetherobacter rufus SBSr003T. Semi-preparative HPLC separations yielded two novel bioactive compounds. Ongoing NMR analysis will enable elucidation of the compounds'; structures. Overall, myxobacteria were exemplified as model organisms for many wide and promising applications.
KW  - Myxobakterien
KW  - Fettsäuren
KW  - Phylogenie
CY  - Saarbrücken
PB  - Universitäts- und Landesbibliothek
AD  - Postfach 151141, 66041 Saarbrücken
UR  - http://scidok.sulb.uni-saarland.de/volltexte/2011/4325
ER  -