TY  - THES
T1  - Interaction of nanoscale particles with the skin barrier
A1  - Labouta,Hagar Ibrahim Mohamed Ibrahim
Y1  - 2011/12/20
N2  - Skin penetration of nanoparticles was the focus of several recent studies. This is of major importance in basic research for potential future applications, e.g. designing topical and transdermal delivery systems, as well as for health risk analysis. Yet, there is a controversy among researchers on the status of their skin penetration due to different experimental setups. Meanwhile, there is little known about the mechanism and determinants of nanoparticle penetration.
The main thesis objective was hence to study the penetration of model gold nanoparticles of different physicochemical and formulation parameters through human skin of different degrees of barrier integrity. Multiphoton microscopy was used for nanoparticle detection. Imaging parameters were determined in terms of resolution and depth profiling of gold nanoparticles in skin. A semiquantitative approach based on pixel analysis of gold nanoparticles was developed to compare nanoparticle localization in different skin locations under different conditions. Based on penetration experiments, determinants that favor or limit particle penetration were determined as well as the barrier to penetration (intercellular lipids). Finally nanoparticle penetration was successfully enhanced using a chemical enhancement approach.
Results obtained are important to enhance our understanding of nanoparticle interaction with the skin barrier. Future studies are required to reduce the gap between research and applications.
KW  - Nanopartikel
KW  - Gold
KW  - Kolloides Metall
KW  - Interaktion
KW  - Haut
KW  - Penetration
CY  - Saarbrücken
PB  - Universitäts- und Landesbibliothek
AD  - Postfach 151141, 66041 Saarbrücken
UR  - http://scidok.sulb.uni-saarland.de/volltexte/2011/4531
ER  -