TY  - THES
T1  - Development of the first metabolite-based LC-MSⁿ urine drug screening procedure
A1  - Wissenbach,Dirk K.
Y1  - 2012/03/30
N2  - In the presented dissertation, the development of the first metabolite-based LC-MS screening approach is described. It consisted of establishing a simple and fast sample workup, fast and sufficient LC separation, MS settings for recording reproducible spectra, and a suitable screening concept. Using these methods, MS2 and MS3 spectra of parent drugs were recorded as well as those of their metabolites after having identified them in urine samples of rats and humans. By using the described methods, the new reference library (over 1,000 parent drugs, 2,700 metabolites, 100 biomolecules), and a sophisticated software tool, a new routine screening approach was established. This LC-MS screening approach is nowerdays an important part of the systematic toxicological analysis and showed excellent robustness and screening results in thousands of authentic patient samples. According to this, this LC-MS approach complements the established GC-MS approach. In addition, recent research projects are partly based on the developed workup, separation and/or detection methods. Finally, this procedure and the reference library could successfully be transferred to another apparatus type, which raised the hope of an instrument independent LC-MS reference library.
KW  - Tandem-Massenspektrometrie
KW  - LC-MS
KW  - Elektronensprayionisations-Massenspektrometrie
KW  - Screening
KW  - Harn
KW  - Metabolit
CY  - Saarbrücken
PB  - Universitäts- und Landesbibliothek
AD  - Postfach 151141, 66041 Saarbrücken
UR  - http://scidok.sulb.uni-saarland.de/volltexte/2012/4797
ER  -