TY  - THES
T1  - Development of early diagnostic systems for ankylosing spondylitis
A1  - Kim,Yeon Joo
Y1  - 2012/07/20
N2  - Ankylosing spondylitis (AS), a prototype of spondyloarthritis (SpA), is a chronic inflammatory arthritis that mainly affects the sarcroiliac joints and the spine. In this study, in order to develop an early diagnosis system for AS, which is known as an autoimmune disease, immunoglobulin variable heavy chain (IgVH) segments were analyzed in peripheral blood mononuclear cells (PBMCs) of patients with AS. We designed three sets of primers (PR1, PR2 and PR3) targeting the unique VH segment. Quantitative PCR (Q-PCR) results revealed that uniquely rearranged VH2 transcripts were about ten times over expressed in AS samples as compared to healthy donor control. This led us to the hypothesis that the paracentric inversion on chromosome 14 and/or class switch recombination deficiency might be associated with AS susceptibility, and this is supported by the overexpression of CD40L.The comparison between relative gene expression amounts in axial AS, healthy donors, and RA patients suggest that newly designed primers could be employed in early diagnosis system development. Future studies will focus on testing the diagnosis of AS by using developed primer sets and the relevance of our findings to AS pathogenesis.
KW  - Spondylitis
KW  - Erstbeschreibung
KW  - Immunglobuline
KW  - Bechterew-Krankheit
CY  - Saarbrücken
PB  - Universitäts- und Landesbibliothek
AD  - Postfach 151141, 66041 Saarbrücken
UR  - http://scidok.sulb.uni-saarland.de/volltexte/2012/4907
ER  -