TY  - THES
T1  - Unraveling the impact of subsurface and surface properties of a material on biological adhesion - a multi-scale approach
A1  - Loskill,Peter Moritz
Y1  - 2012/11/08
N2  - Understanding the adhesion of biological objects to inorganic surfaces is an important research objective in physics and the life sciences. To characterize biological adhesion, most studies describe a substrate solely by its surface properties; the composition of the material beneath the surface is frequently overlooked. That way, long-range van der Waals (vdW) interactions are disregarded. This work reveals that biological objects of all scales—nanoscopic proteins, microscopic bacteria, and macroscopic geckos—are influenced by nanoscale differences in the interface potential. By using tailored silicon wafers with a variable silicon oxide layer thickness, the vdW part of the interface potential is tuned independently from the surface properties. By modifying the wafers with silane monolayers, the surface chemistry can be varied separately as well. On these model substrates, adsorption and adhesion experiments were performed. Protein adsorption was investigated by in situ X-ray reflectometry, bacterial adhesion was explored via AFM force spectroscopy with bacterial probes, and gecko adhesion was characterized using a mechanical testing platform. Moreover, this work investigates whether or not bacterial adhesion is influenced by changes in surface properties such as the fluoridation of artificial teeth or contact-induced rearrangements in the bacterial cell wall and whether or not a reduction of the peptidoglycan crosslinking affects the elasticity of the bacterial cell wall.
KW  - Zelladhäsion
KW  - Adhäsion
KW  - Tokee
KW  - Staphylococcus
KW  - Rasterkraftmikroskopie
KW  - Van-der-Waals-Kraft
KW  - Adsorption
KW  - Biofilm
KW  - Bioadhäsion
CY  - Saarbrücken
PB  - Universitäts- und Landesbibliothek
AD  - Postfach 151141, 66041 Saarbrücken
UR  - http://scidok.sulb.uni-saarland.de/volltexte/2012/4986
ER  -