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doi:10.22028/D291-34248
Title: | Cytoskeletal vimentin regulates cell size and autophagy through mTORC1 signaling |
Author(s): | Mohanasundaram, Ponnuswamy Rato, Leila S. Coelho Modi, Mayank Urbanska, Marta Lautenschläger, Franziska Cheng, Fang Eriksson, John E. |
Language: | English |
Publisher/Platform: | bioRxiv |
Year of Publication: | 2021 |
Publikation type: | Other |
Abstract: | The nutrient-activated mTORC1 (mechanistic target of rapamycin kinase complex 1) signaling pathway determines cell size by controlling mRNA translation, ribosome biogenesis, protein synthesis, and autophagy. Here we show that vimentin, a cytoskeletal intermediate filament protein that we know to be important for wound healing and cancer progression, determines cell size through mTORC1 signaling, an effect that is also manifested at the organism level in mice. We found that vimentin maintains normal cell size by supporting mTORC1 activation and through inhibition of autophagic flux. This regulation is manifested at all levels of downstream target activation and regulation of protein synthesis. We show that vimentin controls mTORC1 mobility by allowing access to lysosomes. Vimentin inhibits the autophagic flux in normal fibroblasts even under starved conditions, indicating a growth factor-independent inhibition of autophagy at the level of mTORC1. Our findings demonstrate that vimentin couples cell size signaling and autophagy with the biomechanic, sensing, and kinetic functions of the cytoskeleton. |
DOI of the first publication: | 10.1101/2021.04.19.440145 |
URL of the first publication: | https://www.biorxiv.org/content/10.1101/2021.04.19.440145v1 |
Link to this record: | hdl:20.500.11880/32121 http://dx.doi.org/10.22028/D291-34248 |
Date of registration: | 4-Jan-2022 |
Notes: | Preprint |
Faculty: | NT - Naturwissenschaftlich- Technische Fakultät |
Department: | NT - Physik |
Professorship: | NT - Jun.-Prof. Dr. Franziska Lautenschläger |
Collections: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
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