Please use this identifier to cite or link to this item: doi:10.22028/D291-34248
Volltext verfügbar? / Dokumentlieferung
Title: Cytoskeletal vimentin regulates cell size and autophagy through mTORC1 signaling
Author(s): Mohanasundaram, Ponnuswamy
Rato, Leila S. Coelho
Modi, Mayank
Urbanska, Marta
Lautenschläger, Franziska
Cheng, Fang
Eriksson, John E.
Language: English
Publisher/Platform: bioRxiv
Year of Publication: 2021
Publikation type: Other
Abstract: The nutrient-activated mTORC1 (mechanistic target of rapamycin kinase complex 1) signaling pathway determines cell size by controlling mRNA translation, ribosome biogenesis, protein synthesis, and autophagy. Here we show that vimentin, a cytoskeletal intermediate filament protein that we know to be important for wound healing and cancer progression, determines cell size through mTORC1 signaling, an effect that is also manifested at the organism level in mice. We found that vimentin maintains normal cell size by supporting mTORC1 activation and through inhibition of autophagic flux. This regulation is manifested at all levels of downstream target activation and regulation of protein synthesis. We show that vimentin controls mTORC1 mobility by allowing access to lysosomes. Vimentin inhibits the autophagic flux in normal fibroblasts even under starved conditions, indicating a growth factor-independent inhibition of autophagy at the level of mTORC1. Our findings demonstrate that vimentin couples cell size signaling and autophagy with the biomechanic, sensing, and kinetic functions of the cytoskeleton.
DOI of the first publication: 10.1101/2021.04.19.440145
URL of the first publication: https://www.biorxiv.org/content/10.1101/2021.04.19.440145v1
Link to this record: hdl:20.500.11880/32121
http://dx.doi.org/10.22028/D291-34248
Date of registration: 4-Jan-2022
Notes: Preprint
Faculty: NT - Naturwissenschaftlich- Technische Fakultät
Department: NT - Physik
Professorship: NT - Jun.-Prof. Dr. Franziska Lautenschläger
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

Files for this record:
There are no files associated with this item.


Items in SciDok are protected by copyright, with all rights reserved, unless otherwise indicated.