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doi:10.22028/D291-41612
Titel: | The Gárdos Channel and Piezo1 Revisited: Comparison between Reticulocytes and Mature Red Blood Cells |
VerfasserIn: | Petkova-Kirova, Polina Murciano, Nicoletta Iacono, Giulia Jansen, Julia Simionato, Greta Qiao, Min Van der Zwaan, Carmen Rotordam, Maria Giustina John, Thomas Hertz, Laura Hoogendijk, Arjan J. Becker, Nadine Wagner, Christian Von Lindern, Marieke Egee, Stephane Van den Akker, Emile Kaestner, Lars |
Sprache: | Englisch |
Titel: | International Journal of Molecular Sciences |
Bandnummer: | 25 |
Heft: | 3 |
Verlag/Plattform: | MDPI |
Erscheinungsjahr: | 2024 |
Freie Schlagwörter: | erythrocytes reticulocytes patch clamp membrane potential NS309 Yoda1 TRAM34 calcium |
DDC-Sachgruppe: | 500 Naturwissenschaften |
Dokumenttyp: | Journalartikel / Zeitschriftenartikel |
Abstract: | The Gárdos channel (KCNN4) and Piezo1 are the best-known ion channels in the red blood cell (RBC) membrane. Nevertheless, the quantitative electrophysiological behavior of RBCs and its heterogeneity are still not completely understood. Here, we use state-of-the-art biochemical methods to probe for the abundance of the channels in RBCs. Furthermore, we utilize automated patch clamp, based on planar chips, to compare the activity of the two channels in reticulocytes and mature RBCs. In addition to this characterization, we performed membrane potential measurements to demonstrate the effect of channel activity and interplay on the RBC properties. Both the Gárdos channel and Piezo1, albeit their average copy number of activatable channels per cell is in the single-digit range, can be detected through transcriptome analysis of reticulocytes. Proteomics analysis of reticulocytes and mature RBCs could only detect Piezo1 but not the Gárdos channel. Furthermore, they can be reliably measured in the whole-cell configuration of the patch clamp method. While for the Gárdos channel, the activity in terms of ion currents is higher in reticulocytes compared to mature RBCs, for Piezo1, the tendency is the opposite. While the interplay between Piezo1 and Gárdos channel cannot be followed using the patch clamp measurements, it could be proved based on membrane potential measurements in populations of intact RBCs. We discuss the Gárdos channel and Piezo1 abundance, interdependencies and interactions in the context of their proposed physiological and pathophysiological functions, which are the passing of small constrictions, e.g., in the spleen, and their active participation in blood clot formation and thrombosis. |
DOI der Erstveröffentlichung: | 10.3390/ijms25031416 |
URL der Erstveröffentlichung: | https://doi.org/10.3390/ijms25031416 |
Link zu diesem Datensatz: | urn:nbn:de:bsz:291--ds-416121 hdl:20.500.11880/37273 http://dx.doi.org/10.22028/D291-41612 |
ISSN: | 1422-0067 |
Datum des Eintrags: | 13-Feb-2024 |
Fakultät: | M - Medizinische Fakultät NT - Naturwissenschaftlich- Technische Fakultät |
Fachrichtung: | M - Anatomie und Zellbiologie NT - Physik |
Professur: | M - Keiner Professur zugeordnet NT - Prof. Dr. Christian Wagner |
Sammlung: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
Dateien zu diesem Datensatz:
Datei | Beschreibung | Größe | Format | |
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ijms-25-01416.pdf | 4,25 MB | Adobe PDF | Öffnen/Anzeigen |
Diese Ressource wurde unter folgender Copyright-Bestimmung veröffentlicht: Lizenz von Creative Commons