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Titel: A Novel Cre Recombinase Mouse Strain for Cell-Specific Deletion of Floxed Genes in Ribbon Synapse-Forming Retinal Neurons
VerfasserIn: Suiwal, Shweta
Wartenberg, Philipp
Boehm, Ulrich
Schmitz, Frank
Schwarz, Karin
Sprache: Englisch
Titel: International Journal of Molecular Sciences
Bandnummer: 25
Heft: 3
Verlag/Plattform: MDPI
Erscheinungsjahr: 2024
Freie Schlagwörter: RIBEYE
retina
photoreceptor
bipolar cells
Cre recombinase
RIBEYE-Cre
ribbon synapse
tau-GFP
DDC-Sachgruppe: 610 Medizin, Gesundheit
Dokumenttyp: Journalartikel / Zeitschriftenartikel
Abstract: We generated a novel Cre mouse strain for cell-specific deletion of floxed genes in ribbon synapse-forming retinal neurons. Previous studies have shown that the RIBEYE promotor targets the expression of recombinant proteins such as fluorescently tagged RIBEYE to photoreceptors and retinal bipolar cells and generates fluorescent synaptic ribbons in situ in these neurons. Here, we used the same promotor to generate a novel transgenic mouse strain in which the RIBEYE promotor controls the expression of a Cre-ER(T2) recombinase (RIBEYE-Cre). To visualize Cre expression, the RIBEYE-Cre animals were crossed with ROSA26 tau-GFP (R26-τGFP) reporter mice. In the resulting RIBEYE-Cre/R26 τGFP animals, Cre-mediated removal of a transcriptional STOP cassette results in the expression of green fluorescent tau protein (tau-GFP) that binds to cellular microtubules. We detected robust tau-GFP expression in retinal bipolar cells. Surprisingly, we did not find fluorescent tau-GFP expression in mouse photoreceptors. The lack of tau-GFP reporter protein in these cells could be based on the previously reported absence of tau protein in mouse photoreceptors which could lead to the degradation of the recombinant tau protein. Consistent with this, we detected Cre and tau-GFP mRNA in mouse photoreceptor slices by RT-PCR. The transgenic RIBEYE-Cre mouse strain provides a new tool to study the deletion of floxed genes in ribbon synapse-forming neurons of the retina and will also allow for analyzing gene deletions that are lethal if globally deleted in neurons.
DOI der Erstveröffentlichung: 10.3390/ijms25031916
URL der Erstveröffentlichung: https://doi.org/10.3390/ijms25031916
Link zu diesem Datensatz: urn:nbn:de:bsz:291--ds-416136
hdl:20.500.11880/37274
http://dx.doi.org/10.22028/D291-41613
ISSN: 1422-0067
Datum des Eintrags: 13-Feb-2024
Fakultät: M - Medizinische Fakultät
Fachrichtung: M - Anatomie und Zellbiologie
M - Experimentelle und Klinische Pharmakologie und Toxikologie
Professur: M - Prof. Dr. Ulrich Boehm
M - Prof. Dr. Frank Schmitz
Sammlung:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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