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doi:10.22028/D291-41613
Titel: | A Novel Cre Recombinase Mouse Strain for Cell-Specific Deletion of Floxed Genes in Ribbon Synapse-Forming Retinal Neurons |
VerfasserIn: | Suiwal, Shweta Wartenberg, Philipp Boehm, Ulrich Schmitz, Frank Schwarz, Karin |
Sprache: | Englisch |
Titel: | International Journal of Molecular Sciences |
Bandnummer: | 25 |
Heft: | 3 |
Verlag/Plattform: | MDPI |
Erscheinungsjahr: | 2024 |
Freie Schlagwörter: | RIBEYE retina photoreceptor bipolar cells Cre recombinase RIBEYE-Cre ribbon synapse tau-GFP |
DDC-Sachgruppe: | 610 Medizin, Gesundheit |
Dokumenttyp: | Journalartikel / Zeitschriftenartikel |
Abstract: | We generated a novel Cre mouse strain for cell-specific deletion of floxed genes in ribbon synapse-forming retinal neurons. Previous studies have shown that the RIBEYE promotor targets the expression of recombinant proteins such as fluorescently tagged RIBEYE to photoreceptors and retinal bipolar cells and generates fluorescent synaptic ribbons in situ in these neurons. Here, we used the same promotor to generate a novel transgenic mouse strain in which the RIBEYE promotor controls the expression of a Cre-ER(T2) recombinase (RIBEYE-Cre). To visualize Cre expression, the RIBEYE-Cre animals were crossed with ROSA26 tau-GFP (R26-τGFP) reporter mice. In the resulting RIBEYE-Cre/R26 τGFP animals, Cre-mediated removal of a transcriptional STOP cassette results in the expression of green fluorescent tau protein (tau-GFP) that binds to cellular microtubules. We detected robust tau-GFP expression in retinal bipolar cells. Surprisingly, we did not find fluorescent tau-GFP expression in mouse photoreceptors. The lack of tau-GFP reporter protein in these cells could be based on the previously reported absence of tau protein in mouse photoreceptors which could lead to the degradation of the recombinant tau protein. Consistent with this, we detected Cre and tau-GFP mRNA in mouse photoreceptor slices by RT-PCR. The transgenic RIBEYE-Cre mouse strain provides a new tool to study the deletion of floxed genes in ribbon synapse-forming neurons of the retina and will also allow for analyzing gene deletions that are lethal if globally deleted in neurons. |
DOI der Erstveröffentlichung: | 10.3390/ijms25031916 |
URL der Erstveröffentlichung: | https://doi.org/10.3390/ijms25031916 |
Link zu diesem Datensatz: | urn:nbn:de:bsz:291--ds-416136 hdl:20.500.11880/37274 http://dx.doi.org/10.22028/D291-41613 |
ISSN: | 1422-0067 |
Datum des Eintrags: | 13-Feb-2024 |
Fakultät: | M - Medizinische Fakultät |
Fachrichtung: | M - Anatomie und Zellbiologie M - Experimentelle und Klinische Pharmakologie und Toxikologie |
Professur: | M - Prof. Dr. Ulrich Boehm M - Prof. Dr. Frank Schmitz |
Sammlung: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
Dateien zu diesem Datensatz:
Datei | Beschreibung | Größe | Format | |
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ijms-25-01916.pdf | 4,01 MB | Adobe PDF | Öffnen/Anzeigen |
Diese Ressource wurde unter folgender Copyright-Bestimmung veröffentlicht: Lizenz von Creative Commons