Please use this identifier to cite or link to this item: doi:10.22028/D291-40022
Title: Cytotoxic effects of different mouthwash solutions on primary human articular chondrocytes and normal human articular cartilage : an in vitro study
Author(s): Cai, Xiaoyu
Venkatesan, Jagadeesh K.
Schmitt, Gertrud
Reda, Bashar
Cucchiarini, Magali
Hannig, Matthias
Madry, Henning
Language: English
Title: Clinical Oral Investigations
Publisher/Platform: Springer Nature
Year of Publication: 2023
Free key words: Articular chondrocyte
Articular cartilage
Human
Mouthwash
Octenidine dihydrochloride
Chlorhexidine gluconate
Toxicity
DDC notations: 610 Medicine and health
Publikation type: Journal Article
Abstract: Objectives To compare the cytotoxicity of octenidine dihydrochloride and chlorhexidine gluconate at diferent concentrations on primary human articular chondrocytes and cartilage. Materials and methods Primary cultures of human normal adult articular chondrocytes were exposed to octenidine dihydrochloride (0.001562%, 0.003125%, 0.00625%, 0.0125%, 0.025%, 0.05%, and 0.1%), chlorhexidine gluconate (0.003125%, 0.00625%, 0.0125%, 0.025%, 0.05%, 0.1%, and 0.2%), and control (Dulbecco’s modifed Eagle medium or phosphate-bufered saline) for 30 s. Normal human articular cartilage explants were exposed to octenidine dihydrochloride (0.1% versus control) and chlorhexidine gluconate (0.1% versus control) for 30 s. The viability of human articular chondrocytes was measured by Trypan blue staining, Cell Proliferation Reagent WST-1, and Live/Dead staining. The proliferation of human chondrocytes was measured using the Cell Proliferation Reagent WST-1. The viability of human articular cartilage explants was measured by using Live/Dead staining. Results Octenidine dihydrochloride and chlorhexidine gluconate exposure decreased cell viability and proliferation in a dose-dependent manner in primary human articular chondrocytes. Octenidine dihydrochloride and chlorhexidine gluconate exposure decreased cell viability in human articular cartilage explant cultures. Conclusion The degree of toxicity varied between octenidine dihydrochloride and chlorhexidine gluconate, with chlorhexidine gluconate being less toxic than octenidine dihydrochloride at the same concentration. Additionally, both octenidine dihydrochloride and chlorhexidine gluconate evaluation had cytotoxic efects on human articular cartilage. Therefore, dosing for the antimicrobial mouthwash ingredients administration would ideally be determined to remain below IC50. Clinical relevance These data support the in vitro safety of antimicrobial mouthwashes on primary adult human articular chondrocytes.
DOI of the first publication: 10.1007/s00784-023-05118-8
URL of the first publication: https://link.springer.com/article/10.1007/s00784-023-05118-8
Link to this record: urn:nbn:de:bsz:291--ds-400228
hdl:20.500.11880/36036
http://dx.doi.org/10.22028/D291-40022
ISSN: 1436-3771
Date of registration: 27-Jun-2023
Faculty: M - Medizinische Fakultät
Department: M - Orthopädie
M - Zahn-, Mund- und Kieferheilkunde
Professorship: M - Prof. Dr. Matthias Hannig
M - Prof. Dr. Henning Madry
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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