Please use this identifier to cite or link to this item:
doi:10.22028/D291-40343
Title: | Protocol to identify amino acids bound to tRNA by aminoacylation using mass spectrometry |
Author(s): | Finkler, Marc Brandt, Joshua Boutfol, Timothée Grimm, Florent Hartz, Philip Ott, Albrecht |
Language: | English |
Title: | STAR Protocols |
Volume: | 4 |
Issue: | 3 |
Publisher/Platform: | Elsevier |
Year of Publication: | 2023 |
Free key words: | Gene Expression Protein Expression and Purification Biotechnology and Bioengineering |
DDC notations: | 500 Science |
Publikation type: | Journal Article |
Abstract: | tRNA-bound amino acids often need to be identified, for instance, in cases where different amino acids compete for binding to the same tRNA. Here, we present a mass-spectrometry-based protocol to determine the amino acids bound to tRNA by aminoacylation. We detail how to perform the aminoacylation reaction, the preparation of the aminoacyl-tRNA for measurement, and the mass spectrometric analysis. We use arginine acylation as an example; however, this protocol can be applied to any other amino acid. |
DOI of the first publication: | 10.1016/j.xpro.2023.102504 |
URL of the first publication: | https://www.sciencedirect.com/science/article/pii/S2666166723004719 |
Link to this record: | urn:nbn:de:bsz:291--ds-403435 hdl:20.500.11880/36292 http://dx.doi.org/10.22028/D291-40343 |
ISSN: | 2666-1667 |
Date of registration: | 21-Aug-2023 |
Faculty: | NT - Naturwissenschaftlich- Technische Fakultät |
Department: | NT - Physik |
Professorship: | NT - Prof. Dr. Albrecht Ott |
Collections: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
Files for this record:
File | Description | Size | Format | |
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1-s2.0-S2666166723004719-main.pdf | 4,11 MB | Adobe PDF | View/Open |
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