Please use this identifier to cite or link to this item:
doi:10.22028/D291-41564
Title: | A tandem of GC‐MS and electroanalysis for a rapid chemical profiling of bacterial extracellular matrix |
Author(s): | Silina, Y. E. Zolotukhina, E. V. Koch, M. Fink‐Straube, C. |
Language: | English |
Title: | Electroanalysis |
Volume: | 35 |
Issue: | 11 |
Publisher/Platform: | Wiley |
Year of Publication: | 2023 |
Free key words: | cyclic voltammetry E. coli extracellular matrix electrochemical profiling Pd-ink-modified electrode |
DDC notations: | 500 Science |
Publikation type: | Journal Article |
Abstract: | Herein an assay toward a rapid and reliable profiling of extracellular matrix of Escherichia coli (E. coli) utilizing a tandem of GC-MS as a tool for definition of the exact chemical nature of low molecular weight compounds and cyclic voltammetry for their high throughput detection is presented. Briefly, during a set of investigations the formation of glycerol in the extracellular matrix (ECM) of E. coli at physiological relevant conditions of cells was revealed. Based on the obtained knowledge, the electrochemical protocol allowing both qualitative and quantitative analyses of glycerol in E. coli ECMs at palladium ink-modified screen printed electrodes with precision values (RSD) <10% and recovery rates ranged from 98% to 102% was proposed. The provided protocol for a rapid electrochemical profiling of the bacterial ECMs can readily be used as a guideline for the controlled electroanalysis of target electroactive signaling analytes in complex biological samples. |
DOI of the first publication: | 10.1002/elan.202300178 |
URL of the first publication: | https://doi.org/10.1002/elan.202300178 |
Link to this record: | urn:nbn:de:bsz:291--ds-415649 hdl:20.500.11880/37251 http://dx.doi.org/10.22028/D291-41564 |
ISSN: | 1521-4109 1040-0397 |
Date of registration: | 6-Feb-2024 |
Faculty: | NT - Naturwissenschaftlich- Technische Fakultät |
Department: | NT - Biowissenschaften |
Professorship: | NT - Prof. Dr. Bruce Morgan |
Collections: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
Files for this record:
File | Description | Size | Format | |
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Electroanalysis - 2023 - Silina - A tandem of GC‐MS and electroanalysis for a rapid chemical profiling of bacterial.pdf | 2,99 MB | Adobe PDF | View/Open |
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