Please use this identifier to cite or link to this item: doi:10.22028/D291-41954
Title: Metabolic Profiling of SH-SY5Y and Neuro2A Cells in Relation to Fetal Calf Serum (FCS) Concentration in Culture Media
Author(s): Kronenberger, Lys
Mett, Janine
Hoppstädter, Jessica
Müller, Uli
Language: English
Title: Metabolites
Volume: 14
Issue: 4
Publisher/Platform: MDPI
Year of Publication: 2024
Free key words: metabolism
physiological cell culture
glucose
glutamine
pyruvate
lactate
SHSY5Y
Neuro2A
DDC notations: 500 Science
Publikation type: Journal Article
Abstract: The neuroblastoma cell lines SH-SY5Y and Neuro2A are commonly utilized models in neurobiological research. DMEM supplemented with different nutrients and 5–10% Fetal Calf Serum (FCS) is typically used for culturing these cell lines. During special treatments, a reduced FCS content is often deployed to reduce cellular proliferation or the content of bioactive compounds. The impact of the reduction of FCS in culture media on the metabolic profile of SH-SY5Y and Neuro2A cells is currently unknown. Using an Amplex Red Assay, this study showed that the consumption of L-glutamine decreased after FCS reduction. Glucose and pyruvate consumption increased in both cell lines after the reduction of FCS. Thus, lactate production also increased with reduced FCS concentration. The reduction of FCS in the cell culture medium resulted in a reduced aerobic ATP production for SH-SY5Y cells and a complete shut down of aerobic ATP production for Neuro2A cells, measured using the Seahorse XF Real-Time ATP Rate Assay. Utilizing the Seahorse XF Glutamine Oxidation Stress Test, Neuro2A cells showed an increased utilization of L-glutamine oxidation after reduction of FCS. These results indicate that changes in FCS concentration in culture media have an impact on the different energy production strategies of SH-SY5Y and Neuro2A cells which must be considered when planning special treatments.
DOI of the first publication: 10.3390/metabo14040188
URL of the first publication: https://doi.org/10.3390/metabo14040188
Link to this record: urn:nbn:de:bsz:291--ds-419542
hdl:20.500.11880/37544
http://dx.doi.org/10.22028/D291-41954
ISSN: 2218-1989
Date of registration: 29-Apr-2024
Description of the related object: Supplementary Materials
Related object: http://www.mdpi.com/article/10.3390/metabo14040188/s1
Faculty: NT - Naturwissenschaftlich- Technische Fakultät
Department: NT - Biowissenschaften
NT - Pharmazie
Professorship: NT - Prof. Dr. Alexandra K. Kiemer
NT - Prof. Dr. Uli Müller
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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