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doi:10.22028/D291-39431
Titel: | High-efficiency production of the antimicrobial peptide pediocin PA-1 in metabolically engineered Corynebacterium glutamicum using a microaerobic process at acidic pH and elevated levels of bivalent calcium ions |
VerfasserIn: | Christmann, Jens Cao, Peng Becker, Judith Desiderato, Christian K. Goldbeck, Oliver Riedel, Christian U. Kohlstedt, Michael Wittmann, Christoph |
Sprache: | Englisch |
Titel: | Microbial Cell Factories |
Bandnummer: | 22 |
Heft: | 1 |
Verlag/Plattform: | BMC |
Erscheinungsjahr: | 2023 |
Freie Schlagwörter: | Corynebacterium glutamicum Pediocin PA-1 Bioprocess Food additive Bacteriocin Antimicrobial peptide Acidic pH Oxygen limitation Calcium supplementation Listeria sp |
DDC-Sachgruppe: | 500 Naturwissenschaften |
Dokumenttyp: | Journalartikel / Zeitschriftenartikel |
Abstract: | Background Pediocin PA-1 is a bacteriocin of recognized value with applications in food bio-preservation and the medical sector for the prevention of infection. To date, industrial manufacturing of pediocin PA-1 is limited by high cost and low-performance. The recent establishment of the biotechnological workhorse Corynebacterium glutamicum as recombinant host for pediocin PA-1 synthesis displays a promising starting point towards more efcient production. Results Here, we optimized the fermentative production process. Following successful simplifcation of the production medium, we carefully investigated the impact of dissolved oxygen, pH value, and the presence of bivalent calcium ions on pediocin production. It turned out that the formation of the peptide was strongly supported by an acidic pH of 5.7 and microaerobic conditions at a dissolved oxygen level of 2.5%. Furthermore, elevated levels of CaCl2 boosted production. The IPTG-inducible producer C. glutamicum CR099 pXMJ19 Ptac pedACDCg provided 66 mg L−1 of pediocin PA-1 in a two-phase batch process using the optimized set-up. In addition, the novel constitutive strain Ptuf pedACDCg allowed successful production without the need for IPTG. Conclusions The achieved pediocin titer surpasses previous eforts in various microbes up to almost seven-fold, providing a valuable step to further explore and develop this important bacteriocin. In addition to its high biosynthetic performance C. glutamicum proved to be highly robust under the demanding producing conditions, suggesting its further use as host for bacteriocin production. |
DOI der Erstveröffentlichung: | 10.1186/s12934-023-02044-y |
URL der Erstveröffentlichung: | https://microbialcellfactories.biomedcentral.com/articles/10.1186/s12934-023-02044-y |
Link zu diesem Datensatz: | urn:nbn:de:bsz:291--ds-394316 hdl:20.500.11880/35551 http://dx.doi.org/10.22028/D291-39431 |
ISSN: | 1475-2859 |
Datum des Eintrags: | 31-Mär-2023 |
Bezeichnung des in Beziehung stehenden Objekts: | Supplementary Information |
In Beziehung stehendes Objekt: | https://static-content.springer.com/esm/art%3A10.1186%2Fs12934-023-02044-y/MediaObjects/12934_2023_2044_MOESM1_ESM.pdf |
Fakultät: | NT - Naturwissenschaftlich- Technische Fakultät |
Fachrichtung: | NT - Biowissenschaften |
Professur: | NT - Prof. Dr. Christoph Wittmann |
Sammlung: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
Dateien zu diesem Datensatz:
Datei | Beschreibung | Größe | Format | |
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s12934-023-02044-y.pdf | 3,05 MB | Adobe PDF | Öffnen/Anzeigen |
Diese Ressource wurde unter folgender Copyright-Bestimmung veröffentlicht: Lizenz von Creative Commons