Please use this identifier to cite or link to this item:
doi:10.22028/D291-39431
Title: | High-efficiency production of the antimicrobial peptide pediocin PA-1 in metabolically engineered Corynebacterium glutamicum using a microaerobic process at acidic pH and elevated levels of bivalent calcium ions |
Author(s): | Christmann, Jens Cao, Peng Becker, Judith Desiderato, Christian K. Goldbeck, Oliver Riedel, Christian U. Kohlstedt, Michael Wittmann, Christoph |
Language: | English |
Title: | Microbial Cell Factories |
Volume: | 22 |
Issue: | 1 |
Publisher/Platform: | BMC |
Year of Publication: | 2023 |
Free key words: | Corynebacterium glutamicum Pediocin PA-1 Bioprocess Food additive Bacteriocin Antimicrobial peptide Acidic pH Oxygen limitation Calcium supplementation Listeria sp |
DDC notations: | 500 Science |
Publikation type: | Journal Article |
Abstract: | Background Pediocin PA-1 is a bacteriocin of recognized value with applications in food bio-preservation and the medical sector for the prevention of infection. To date, industrial manufacturing of pediocin PA-1 is limited by high cost and low-performance. The recent establishment of the biotechnological workhorse Corynebacterium glutamicum as recombinant host for pediocin PA-1 synthesis displays a promising starting point towards more efcient production. Results Here, we optimized the fermentative production process. Following successful simplifcation of the production medium, we carefully investigated the impact of dissolved oxygen, pH value, and the presence of bivalent calcium ions on pediocin production. It turned out that the formation of the peptide was strongly supported by an acidic pH of 5.7 and microaerobic conditions at a dissolved oxygen level of 2.5%. Furthermore, elevated levels of CaCl2 boosted production. The IPTG-inducible producer C. glutamicum CR099 pXMJ19 Ptac pedACDCg provided 66 mg L−1 of pediocin PA-1 in a two-phase batch process using the optimized set-up. In addition, the novel constitutive strain Ptuf pedACDCg allowed successful production without the need for IPTG. Conclusions The achieved pediocin titer surpasses previous eforts in various microbes up to almost seven-fold, providing a valuable step to further explore and develop this important bacteriocin. In addition to its high biosynthetic performance C. glutamicum proved to be highly robust under the demanding producing conditions, suggesting its further use as host for bacteriocin production. |
DOI of the first publication: | 10.1186/s12934-023-02044-y |
URL of the first publication: | https://microbialcellfactories.biomedcentral.com/articles/10.1186/s12934-023-02044-y |
Link to this record: | urn:nbn:de:bsz:291--ds-394316 hdl:20.500.11880/35551 http://dx.doi.org/10.22028/D291-39431 |
ISSN: | 1475-2859 |
Date of registration: | 31-Mar-2023 |
Description of the related object: | Supplementary Information |
Related object: | https://static-content.springer.com/esm/art%3A10.1186%2Fs12934-023-02044-y/MediaObjects/12934_2023_2044_MOESM1_ESM.pdf |
Faculty: | NT - Naturwissenschaftlich- Technische Fakultät |
Department: | NT - Biowissenschaften |
Professorship: | NT - Prof. Dr. Christoph Wittmann |
Collections: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
Files for this record:
File | Description | Size | Format | |
---|---|---|---|---|
s12934-023-02044-y.pdf | 3,05 MB | Adobe PDF | View/Open |
This item is licensed under a Creative Commons License